Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A301-320A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSF/SFPQ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PSF/SFPQ by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody (Product # A301-320A; lot A301-320A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 5 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PSF/SFPQ by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody A301-320A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies A301-321A and A301-322A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 5 seconds (A) and 1 second (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PSF/SFPQ by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PSF/SFPQ (Cat. No. A301-320A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PSF/SFPQ by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PSF/SFPQ antibody (Product # A301-320A; lot A301-320A-1) used for IP at 3 µg/mg lysate. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies (Product # A301-321A and Product # A301-322A), which recognize downstream epitopes. For blotting immunoprecipitated PSF/SFPQ (Product # A301-320A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 1 second.
