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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB4782 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4782, RRID:AB_1577349
- Product name
- MMP14 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MMP14.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas.
cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
Cloning of a human gene potentially encoding a novel matrix metalloproteinase having a C-terminal transmembrane domain.
Okada A, Bellocq JP, Rouyer N, Chenard MP, Rio MC, Chambon P, Basset P
Proceedings of the National Academy of Sciences of the United States of America 1995 Mar 28;92(7):2730-4
Proceedings of the National Academy of Sciences of the United States of America 1995 Mar 28;92(7):2730-4
cDNA sequence and mRNA tissue distribution of a novel human matrix metalloproteinase with a potential transmembrane segment.
Will H, Hinzmann B
European journal of biochemistry / FEBS 1995 Aug 1;231(3):602-8
European journal of biochemistry / FEBS 1995 Aug 1;231(3):602-8
Cloning of a human gene potentially encoding a novel matrix metalloproteinase having a C-terminal transmembrane domain.
Takino T, Sato H, Yamamoto E, Seiki M
Gene 1995 Apr 3;155(2):293-8
Gene 1995 Apr 3;155(2):293-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The MMP14 polyclonal antibody (Cat # PAB4782) is used in Western blot to detect MMP14 in A-375 cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MMP14 (arrow) using rabbit MMP14 polyclonal antibody (Cat # PAB4782). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MMP14 polyclonal antibody (Cat # PAB4782) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using MMP14 polyclonal antibody (Cat # PAB4782)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
