M00656

antibody from Boster Biological Technology

Targeting: MMP14 MT1-MMP

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00656

Provider

Boster Biological Technology

Product name

Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for MT1 MMP/MMP14 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat MT1 MMP/MMP14 information: Molecular Weight: 65894 MW; Subcellular Localization: Membrane ; Single-pass type I membrane protein . Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm; Tissue Specificity: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

BFD-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human kdieny, using MMP14 Antibody (M00656)MMP14 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MMP14 Antibody (M00656)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image