Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-32076 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MMP14 Recombinant Rabbit Monoclonal Antibody (SJ18-09)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SJ18-09
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Kallikrein-Related Peptidase 14 Activates Zymogens of Membrane Type Matrix Metalloproteinases (MT-MMPs)-A CleavEx Based Analysis.
Falkowski K, Bielecka E, Thøgersen IB, Bocheńska O, Płaza K, Kalińska M, Sąsiadek L, Magoch M, Pęcak A, Wiśniewska M, Gruba N, Wysocka M, Wojtysiak A, Brzezińska-Bodal M, Sychowska K, Pejkovska A, Rehders M, Butler G, Overall CM, Brix K, Dubin G, Lesner A, Kozik A, Enghild JJ, Potempa J, Kantyka T
International journal of molecular sciences 2020 Jun 19;21(12)
International journal of molecular sciences 2020 Jun 19;21(12)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MMP14 in human kidney tissue lysate using a MMP14 Monoclonal antibody (Product # MA5-32076) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of MMP14 in CRC cells using a MMP14 Monoclonal antibody (Product # MA5-32076) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of MMP14 in BT-20 cells using a MMP14 Monoclonal antibody (Product # MA5-32076) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP14 of paraffin-embedded Human breast carcinoma tissue using a MMP14 Monoclonal antibody (Product #MA5-32076). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP14 of paraffin-embedded Human kidney tissue using a MMP14 Monoclonal antibody (Product #MA5-32076). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MMP14 of paraffin-embedded Human uterus tissue using a MMP14 Monoclonal antibody (Product #MA5-32076). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of MMP14 in A549 cells using a MMP14 Monoclonal Antibody (Product # MA5-32076) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Processing of cell surface proMMP14 by KLK14. Murine fibroblasts stably expressing human MMP14 (MT1-MMP) were treated with KLK14 and furin. Selective inhibitors serine protease inhibitor Kazal-type 6 (SPINK6) (KLK14) and dec-RVKR-CMK (furin) were used to inhibit KLK and furin in the control samples. Cell surface proteins were then biotinylated, and streptavidin bead immunoprecipitates were subjected to immunoblotting using an anti-MMP14 antibody. Each sample contained the 63 kDa proMMP14 form, whereas an increase in the active 58 kDa MMP14 form was observed after KLK14 incubation. Additionally, a lower molecular weight MMP14 form at 56 kDa was detected only in the KLK14 treated sample. KLK = kallikrein-related peptidase; MMP = matrix metalloproteinase.