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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX108567 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108567, RRID:AB_1951092
- Product name
- PACSIN1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE GTX108567 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 £gg of whole cell lysate)A:MOLT4(GTX27912)7.5% SDS PAGEGTX108567 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PACSIN1 antibody (GTX108567) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PACSIN1 antibody detects PACSIN1 protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: PACSIN1 protein stained by PACSIN1 antibody (GTX108567) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PACSIN1 antibody detects PACSIN1 protein at cytoplasm on MCF7 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded MCF7 xenograft. PACSIN1 antibody (GTX108567) dilution: 1:500.
