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Learn12957-1-AP
antibody from Invitrogen Antibodies
Targeting: DNM1L
DRP1, DVLP, DYMPLE, HDYNIV, VPS1
Western blot Immunocytochemistry
Immunoprecipitation
Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 12957-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DRP1 (C-terminal) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: CGGARICYI FHETFGRTLE SVDPLGGLNT IDILTAIRNA TGPRPALFVP EVSFELLVKR QIKRLEEPSL RCVELVHEEM QRIIQHCSNY STQELLRFPK LHDAIVEVVT CLLRKRLPVT NEMVHNLVAI ELAYINTKHP DFADACGLMN NNIEEQRRNR LARELPSAVS RDKLIQDSRR ETKNVASGGG GVGDGVQEPT TGNWRGMLKT SKAEELLAEE KSKPIPIMPA SPQKGHAVNL LDVPVPVARK LSAREQRDCE VIERLIKSYF LIVRKNIQDS VPKAVMHFLV NHVKDTLQSE LVGQLYKSSL LDDLLTESED MAQRRKEAAD MLKALQGASQ IIAEIRETHL W (361-711 aa encoded by BC024590)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.16 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12957-1-AP ( DRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12957-1-AP (DRP1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12957-1-AP (DRP1 (C-terminal) antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human DRP1 (C-terminal) (Product # 12957-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DRP1 (IP:12957-1-AP, 4ug; Detection:12957-1-AP 1:500) with mouse brain tissue lysate 3600ug.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-DRP1 (C-terminal) (IP:12957-1-AP, 4 ug; Detection:12957-1-AP 1:1000) with mouse brain tissue lysate 2400 ug.
