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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-380 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-380, RRID:AB_2263466
- Product name
- Anti-GNB2
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Yu MJ, Pisitkun T, Wang G, Aranda JF, Gonzales PA, Tchapyjnikov D, Shen RF, Alonso MA, Knepper MA
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.
Lobanova ES, Finkelstein S, Herrmann R, Chen YM, Kessler C, Michaud NA, Trieu LH, Strissel KJ, Burns ME, Arshavsky VY
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3510-20
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3510-20
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 12;103(37):13759-64
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 12;103(37):13759-64
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 37
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
