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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-393 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-393, RRID:AB_631536
- Product name
- Anti-GNAQ
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Interplay between the heterotrimeric G-protein subunits Galphaq and Galphai2 sets the threshold for chemotaxis and TCR activation.
Disruption of ROCK1 gene attenuates cardiac dilation and improves contractile function in pathological cardiac hypertrophy.
Negative regulation of parathyroid hormone (PTH)-activated phospholipase C by PTH/PTH-related peptide receptor phosphorylation and protein kinase A.
Wnt signalling regulates paxillin ubiquitination essential for mesodermal cell motility
Human cytomegalovirus-encoded G protein-coupled receptor US28 mediates smooth muscle cell migration through Galpha12.
Segregation of heterotrimeric G proteins in cell surface microdomains. G(q) binds caveolin to concentrate in caveolae, whereas G(i) and G(s) target lipid rafts by default.
Ngai J, Inngjerdingen M, Berge T, Taskén K
BMC immunology 2009 May 8;10:27
BMC immunology 2009 May 8;10:27
Disruption of ROCK1 gene attenuates cardiac dilation and improves contractile function in pathological cardiac hypertrophy.
Shi J, Zhang YW, Summers LJ, Dorn GW 2nd, Wei L
Journal of molecular and cellular cardiology 2008 Mar;44(3):551-60
Journal of molecular and cellular cardiology 2008 Mar;44(3):551-60
Negative regulation of parathyroid hormone (PTH)-activated phospholipase C by PTH/PTH-related peptide receptor phosphorylation and protein kinase A.
Tawfeek HA, Abou-Samra AB
Endocrinology 2008 Aug;149(8):4016-23
Endocrinology 2008 Aug;149(8):4016-23
Wnt signalling regulates paxillin ubiquitination essential for mesodermal cell motility
Hidekazu Iioka, Shun-ichiro Iemura, Tohru Natsume, Noriyuki Kinoshita
Nature Cell Biology 2007 Jun;9(7):813-821
Nature Cell Biology 2007 Jun;9(7):813-821
Human cytomegalovirus-encoded G protein-coupled receptor US28 mediates smooth muscle cell migration through Galpha12.
Melnychuk RM, Streblow DN, Smith PP, Hirsch AJ, Pancheva D, Nelson JA
Journal of virology 2004 Aug;78(15):8382-91
Journal of virology 2004 Aug;78(15):8382-91
Segregation of heterotrimeric G proteins in cell surface microdomains. G(q) binds caveolin to concentrate in caveolae, whereas G(i) and G(s) target lipid rafts by default.
Oh P, Schnitzer JE
Molecular biology of the cell 2001 Mar;12(3):685-98
Molecular biology of the cell 2001 Mar;12(3):685-98
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 42
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
