13927-1-AP

antibody from Invitrogen Antibodies

Targeting: GNAQ G-ALPHA-q, GAQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

13927-1-AP

Provider

Invitrogen Antibodies

Product name

GNAQ Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTLESIMAC CLSEEAKEAR RINDEIERQL RRDKRDARRE LKLLLLGTGE SGKSTFIKQM RIIHGSGYSD EDKRGFTKLV YQNIFTAMQA MIRAMDTLKI PYKYEHNKAH AQLVREVDVE KVSAFENPYV DAIKSLWNDP GIQECYDRRR EYQLSDSTKY YLNDLDRVAD PAYLPTQQDV LRVRVPTTGI IEYPFDLQSV IFRMVDVGGQ RSERRKWIHC FENVTSIMFL VALSEYDQVL VESDNENRME ESKALFRTII TYPWFQNSSV ILFLNKKDLL EEKIMYSHLV DYFPEYDGPQ RDAQAAREFI LKMFVDLNPD SDKIIYSHFT CATDTENIRF VFAAVKDTIL QLNLKEYNLV (1-359 aa encoded by BC057777)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13927-1-AP (GNAQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed HEK-293 cells using 13927-1-AP (GNAQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13927-1-AP (GNAQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).