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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 629201 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#629201, RRID:AB_493419
- Product name
- Purified anti-Tubulin-
- Antibody type
- Monoclonal
- Antigen
- Peptide-KLH
- Description
- γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Cll extract from A431 cells (Lane 1) or NIH3T3 cells (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-γ-tubulin (Clone 14C11) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
