PA5-17790

antibody from Invitrogen Antibodies

Targeting: PLA2G4A cPLA2-alpha, PLA2G4

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-17790

Provider

Invitrogen Antibodies

Product name

Phospho-PLA2G4A (Ser505) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

250 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PLA2G4A Polyclonal Antibody (Product # PA5-17790) and a ~85 kDa band was observed across the panel tested except IMR-32 and Reh. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A549 (Lane 2), NIH/3T3 (Lane 3), IMR-32 (Lane 4) and Reh (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).