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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP1-52050 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52050, RRID:AB_11024402
- Product name
- Goat Polyclonal PLA2G4A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-QEKTFRQQRKEHIR- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references JNK and ceramide kinase govern the biogenesis of lipid droplets through activation of group IVA phospholipase A2.
Gubern A, Barceló-Torns M, Barneda D, López JM, Masgrau R, Picatoste F, Chalfant CE, Balsinde J, Balboa MA, Claro E
The Journal of biological chemistry 2009 Nov 20;284(47):32359-69
The Journal of biological chemistry 2009 Nov 20;284(47):32359-69
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PLA2G4A Antibody [NBP1-52050] - A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PLA2G4A Antibody [NBP1-52050] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PLA2G4A Antibody [NBP1-52050] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
