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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF7769 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Midkine Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Midkine in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Midkine is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P12025- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Midkine by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Midkine at approximately 15-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Midkine by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Midkine at approximately 26 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Midkine in Mouse Embryo. Midkine was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium in ribs. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
