M01823

antibody from Boster Biological Technology

Targeting: MDK FLJ27379, MK, NEGF2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01823

Provider

Boster Biological Technology

Product name

Anti-Midkine MDK Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Midkine/MDK detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IF, IHC, ICC, WB. Reactive species: Human Midkine/MDK information: Molecular Weight: 15585 MW; Subcellular Localization: Secreted; Tissue Specificity: Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.

Reactivity

Human

Host

Rabbit

Antibody clone number

EBI-13

Vial size

100ug/vial

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human liver carcinoma, using Midkine Antibody (M01823)MDK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MDK Antibody (M01823)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image