- GTX107614 - GeneTex PGK1 antibody

Submitted references Inflammatory interferon activates HIF-1α-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway.

Yeh YH, Hsiao HF, Yeh YC, Chen TW, Li TK
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):70

Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.

Chang SJ, Liao EC, Yeo HY, Kuo WH, Chen HY, Tsai YT, Wei YS, Chen YJ, Wang YS, Li JM, Shih CC, Chan CH, Lai ZY, Chou HC, Chuang YJ, Chan HL
Archives of biochemistry and biophysics 2018 Jun 1;647:10-32

Branched-chain ketoacids secreted by glioblastoma cells via MCT1 modulate macrophage phenotype.

Silva LS, Poschet G, Nonnenmacher Y, Becker HM, Sapcariu S, Gaupel AC, Schlotter M, Wu Y, Kneisel N, Seiffert M, Hell R, Hiller K, Lichter P, Radlwimmer B
EMBO reports 2017 Dec;18(12):2172-2185

Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

Lin LL, Hsia CR, Hsu CL, Huang HC, Juan HF
BMC genomics 2015 Feb 5;16:41

The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.

Drusenheimer N, Migdal B, Jäckel S, Tveriakhina L, Scheider K, Schulz K, Gröper J, Köhrer K, Klein T
PLoS genetics 2015 Dec;11(12):e1005749

The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.

Lee SO, Jin UH, Kang JH, Kim SB, Guthrie AS, Sreevalsan S, Lee JS, Safe S
Molecular cancer research : MCR 2014 Apr;12(4):527-538

Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

Wu YH, Hu CW, Chien CW, Chen YJ, Huang HC, Juan HF
PloS one 2013;8(8):e70642

High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.

Chen JY, Chou HC, Chen YH, Chan HL
The Journal of nutritional biochemistry 2013 Nov;24(11):1889-910

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis. Upper panel: PGK1 antibody (GTX107614) Lower panel: beta Actin antibody (GTX109639) A. 30 ?g HeLa whole cell lysate/extract (untreated) B. 30 ?g HeLa whole cell lysate/extract (1% O2 treatment for 24hr) 10% SDS PAGE? PGK1 antibody (GTX107614) dilution: 1:1000 beta Actin antibody (GTX109639) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX107614 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: PGK1 antibody detects PGK1 protein by western blot analysis. A. 50 ?g Rat liver lysate/extract 10% SDS-PAGE PGK1 antibody (GTX107614) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A549 B: Hela 10% SDS PAGE GTX107614 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.

GTX107614

Antibody data