Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 654102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#654102, RRID:AB_2561782
- Product name
- Purified anti-TIF1ß (KAP-1, TRIM28) Phospho (Ser473)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide: VKRSRpSGEGEVC.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 11G10SC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Hela cell extracts untreated (lane 1) and treated with UV radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β phosphorylated (Ser473) antibody (clone 11G10SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated