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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- R34961 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Beta CstF-64 Antibody (variants 1 & 3)
- Antibody type
- Polyclonal
- Antigen
- Amino acids PASIERVQGQRT were used as the immunogen for this beta CstF-64 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the beta CstF-64 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot: HeLa lysate either in RIPA (lanes 1, 2, 4, 6) or TX100 buffer (lanes 3, 5, 7) overexpressing mouse beta Cstf-64-FLAG immunoprecipitated using beta Cstf-64 (2ug) antibody in lanes 6 & 7 and probed with anti-FLAG [1/1000]. Mock-transfected HeLa in Lane 1.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation: Beta CstF-64 antibody is able to pull down bCstf-64 from mouse brain lysate (lane 5, 500ug protein) but not Cstf-64 from mouse liver lysate (lane 6, 500ug protein) using protein G-coated magnetic beads. WB performed with unrelated bCstf-64/Cstf-64 mAb.
