NB100-57530

antibody from Novus Biologicals

Targeting: CSTF2 CstF-64

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-57530

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-57530, RRID:AB_921071

Product name

Rabbit Polyclonal Cleavage stimulation factor 2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of Human CSTF64 on HeLa whole cell lysate (5, 15, and 50 microgram) using NB100-57530. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57531.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human and mouse Cleavage stimulation factor 2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 (Cleavage stimulation factor 2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CSTF64 antibody NB100-57530 used for IP at 3 ug per reaction. CSTF64 was also immunoprecipitated by rabbit anti-CSTF64 antibody NB100-57531. For blotting immunoprecipitated CSTF64, NB100-57530 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CSTF64 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Cleavage stimulation factor 2 Antibody [NB100-57530] - Detection of human CSTF64 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CSTF64 (NB100-57530) used at a dilution of 1:200 (1ug/ml). Detection: DAB