Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- MA5-32587 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S1P1 Recombinant Rabbit Monoclonal Antibody (JM10-66)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM10-66
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S1P1 in SH-SY5Y cell lysate using a S1P1 Monoclonal antibody (Product # MA5-32587) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S1P1 in SH-SY5Y cell lysate using a S1P1 Monoclonal antibody (Product # MA5-32587) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of S1P1 in HepG2 cells using a S1P1 Monoclonal antibody (Product # MA5-32587) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of S1P1 in HUVEC cells using a S1P1 Monoclonal antibody (Product # MA5-32587) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of S1P1 in SH-SY5Y cells using a S1P1 Monoclonal antibody (Product # MA5-32587) as seen in red. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S1P1 of paraffin-embedded Human breast carcinoma tissue using a S1P1 Monoclonal antibody (Product #MA5-32587). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S1P1 of paraffin-embedded Human liver tissue using a S1P1 Monoclonal antibody (Product #MA5-32587). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S1P1 of paraffin-embedded Human kidney tissue using a S1P1 Monoclonal antibody (Product #MA5-32587). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S1P1 of paraffin-embedded Mouse brain tissue using a S1P1 Monoclonal antibody (Product #MA5-32587). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S1P1 of paraffin-embedded rat tissue using a S1P1 Monoclonal antibody (Product #MA5-32587). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of S1P1 in Jurkat cells using a S1P1 Monoclonal Antibody (Product # MA5-32587) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.