3738

antibody from Cell Signaling Technology, Inc

Targeting: BECN1 ATG6, VPS30

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

3738

Provider

Cell Signaling Technology, Inc

Proper citation

Cell Signaling Technology Cat#3738, RRID:AB_490837

Product name

Beclin-1 Antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

-20°C

References

Submitted references

Beclin-1 expression is retained in high-grade serous ovarian cancer yet is not essential for autophagy induction in vitro.

Correa RJ, Valdes YR, Shepherd TG, DiMattia GE
Journal of ovarian research 2015 Aug 4;8:52

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Combination of AKT inhibition with autophagy blockade effectively reduces ascites-derived ovarian cancer cell viability.

Correa RJ, Valdes YR, Peart TM, Fazio EN, Bertrand M, McGee J, Préfontaine M, Sugimoto A, DiMattia GE, Shepherd TG
Carcinogenesis 2014 Sep;35(9):1951-61

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GSK-3beta promotes cell survival by modulating Bif-1-dependent autophagy and cell death.

Yang J, Takahashi Y, Cheng E, Liu J, Terranova PF, Zhao B, Thrasher JB, Wang HG, Li B
Journal of cell science 2010 Mar 15;123(Pt 6):861-70

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MyD88 and Trif target Beclin 1 to trigger autophagy in macrophages.

Shi CS, Kehrl JH
The Journal of biological chemistry 2008 Nov 28;283(48):33175-82

Western blot

Supportive validation

Submitted by

561fce546b842

Main image

Experimental details

Beclin-1 (cell signaling 3738) 1:500 in 5%milk+TBST, 2nd 6000R, 60Kd

Sample type

Cell Lines

Validation comment

Good

Primary Ab dilution

1:500

Conjugate

Unconjugated

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:6000