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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10500 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10500, RRID:AB_11189369
- Product name
- BECN1 monoclonal antibody, clone 2A4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant BECN1.
- Isotype
- IgG
- Antibody clone number
- 2A4
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) against HeLa (1), A-431 (2), MCF-7 (3), Raji (4), Jurkat (5) and SK-BR-3 (6) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA detection with BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (A) and liver cancer tissues (B) using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Raji cells using BECN1 monoclonal antibody, clone 2A4 (Cat # MAB10500) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
