Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG54959 - Provider product page
- Provider
- Arigo
- Product name
- anti-Beclin-1 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified His-tagged recombinant full length Human Autophagy Beclin-1.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 15CT26
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2, 293, Jurkat and HeLa cell line lysates stained with ARG54959 anti-Beclin-1 antibody at 1:2000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: recombinant BECN1 protein stained with ARG54959 anti-Beclin-1 antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cells stained with ARG54959 anti-Beclin-1 antibody (green) at 1:100 dilution, 2 h at room temperature. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54959 anti-Beclin-1 antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: Breast CA section stained with ARG54959 anti-Beclin-1 antibody at 1:50 dilution.
