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- Antibody Data
- Antigen structure
- References [42]
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- Western blot [5]
- Immunohistochemistry [4]
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- Product number
- NB110-87318 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-87318, RRID:AB_1201249
- Product name
- Rabbit Polyclonal Beclin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Canine, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Detection and analysis of autophagy in the American alligator (Alligator mississippiensis).
Induction of autophagy and suppression of type I IFN secretion by CSFV.
SIRT3 deficiency is resistant to autophagy-dependent ferroptosis by inhibiting the AMPK/mTOR pathway and promoting GPX4 levels.
Human immunodeficiency virus Type-1 single-stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia.
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
Liver ASK1 protects from non-alcoholic fatty liver disease and fibrosis.
ROS-induced autophagy regulates porcine trophectoderm cell apoptosis, proliferation, and differentiation.
T-Type Cav3.1 Channels Mediate Progression and Chemotherapeutic Resistance in Glioblastoma.
Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers.
TGFB1 is secreted through an unconventional pathway dependent on the autophagic machinery and cytoskeletal regulators.
Cell Specific Changes of Autophagy in a Mouse Model of Contusive Spinal Cord Injury.
Oleuropein attenuates hydrogen peroxide-induced autophagic cell death in human adipose-derived stem cells.
ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation.
MicroRNA-101 Modulates Autophagy and Oligodendroglial Alpha-Synuclein Accumulation in Multiple System Atrophy.
Negative LC3b immunoreactivity in cancer cells is an independent prognostic predictor of prostate cancer specific death.
IRE1α promotes viral infection by conferring resistance to apoptosis.
Ginsenoside Rg1 protects against sepsis-associated encephalopathy through beclin 1-independent autophagy in mice.
Human Rad23A plays a regulatory role in autophagy.
Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells.
Amber Light (590 nm) Induces the Breakdown of Lipid Droplets through Autophagy-Related Lysosomal Degradation in Differentiated Adipocytes.
Hinokitiol induces autophagy in murine breast and colorectal cancer cells.
Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease.
A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition.
Interference of Notch1 inhibits the growth of glioma cancer cells by inducing cell autophagy and down-regulation of Notch1-Hes-1 signaling pathway.
In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy.
Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine.
Sirtuin 1 (SIRT1) Deacetylase Activity and NAD⁺/NADH Ratio Are Imperative for Capsaicin-Mediated Programmed Cell Death.
Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine.
Autophagy enhances the replication of classical swine fever virus in vitro.
HMGB1 is involved in autophagy inhibition caused by SNCA/α-synuclein overexpression: a process modulated by the natural autophagy inducer corynoxine B.
Maintenance of basal levels of autophagy in Huntington's disease mouse models displaying metabolic dysfunction.
BECN1/Beclin 1 is recruited into lipid rafts by prion to activate autophagy in response to amyloid β 42.
BECN1/Beclin 1 is recruited into lipid rafts by prion to activate autophagy in response to amyloid β 42.
Influenza A virus proteins NS1 and hemagglutinin along with M2 are involved in stimulation of autophagy in infected cells.
Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing.
8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers.
PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.
Presenilin is necessary for efficient proteolysis through the autophagy-lysosome system in a γ-secretase-independent manner.
Profound cardioprotection with chloramphenicol succinate in the swine model of myocardial ischemia-reperfusion injury.
Hypoxia-induced autophagy is mediated through hypoxia-inducible factor induction of BNIP3 and BNIP3L via their BH3 domains.
Effect of autophagy on multiple myeloma cell viability.
Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages.
Hale A, Merchant M, White M
Journal of experimental zoology. Part B, Molecular and developmental evolution 2020 May;334(3):192-207
Journal of experimental zoology. Part B, Molecular and developmental evolution 2020 May;334(3):192-207
Induction of autophagy and suppression of type I IFN secretion by CSFV.
Xie B, Zhao M, Song D, Wu K, Yi L, Li W, Li X, Wang K, Chen J
Autophagy 2020 Mar 21;:1-23
Autophagy 2020 Mar 21;:1-23
SIRT3 deficiency is resistant to autophagy-dependent ferroptosis by inhibiting the AMPK/mTOR pathway and promoting GPX4 levels.
Han D, Jiang L, Gu X, Huang S, Pang J, Wu Y, Yin J, Wang J
Journal of cellular physiology 2020 Apr 24;
Journal of cellular physiology 2020 Apr 24;
Human immunodeficiency virus Type-1 single-stranded RNA activates the NLRP3 inflammasome and impairs autophagic clearance of damaged mitochondria in human microglia.
Rawat P, Teodorof-Diedrich C, Spector SA
Glia 2019 May;67(5):802-824
Glia 2019 May;67(5):802-824
Network analysis and mechanisms of action of Chinese herb-related natural compounds in lung cancer cells.
Lin YJ, Liang WM, Chen CJ, Tsang H, Chiou JS, Liu X, Cheng CF, Lin TH, Liao CC, Huang SM, Chen J, Tsai FJ, Li TM
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
Phytomedicine : international journal of phytotherapy and phytopharmacology 2019 May;58:152893
Liver ASK1 protects from non-alcoholic fatty liver disease and fibrosis.
Challa TD, Wueest S, Lucchini FC, Dedual M, Modica S, Borsigova M, Wolfrum C, Blüher M, Konrad D
EMBO molecular medicine 2019 Jun 6;
EMBO molecular medicine 2019 Jun 6;
ROS-induced autophagy regulates porcine trophectoderm cell apoptosis, proliferation, and differentiation.
Luo Z, Xu X, Sho T, Zhang J, Xu W, Yao J, Xu J
American journal of physiology. Cell physiology 2019 Feb 1;316(2):C198-C209
American journal of physiology. Cell physiology 2019 Feb 1;316(2):C198-C209
T-Type Cav3.1 Channels Mediate Progression and Chemotherapeutic Resistance in Glioblastoma.
Visa A, Sallán MC, Maiques O, Alza L, Talavera E, López-Ortega R, Santacana M, Herreros J, Cantí C
Cancer research 2019 Apr 15;79(8):1857-1868
Cancer research 2019 Apr 15;79(8):1857-1868
Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers.
Nàger M, Sallán MC, Visa A, Pushparaj C, Santacana M, Macià A, Yeramian A, Cantí C, Herreros J
Autophagy 2018;14(4):619-636
Autophagy 2018;14(4):619-636
TGFB1 is secreted through an unconventional pathway dependent on the autophagic machinery and cytoskeletal regulators.
Nüchel J, Ghatak S, Zuk AV, Illerhaus A, Mörgelin M, Schönborn K, Blumbach K, Wickström SA, Krieg T, Sengle G, Plomann M, Eckes B
Autophagy 2018;14(3):465-486
Autophagy 2018;14(3):465-486
Cell Specific Changes of Autophagy in a Mouse Model of Contusive Spinal Cord Injury.
Muñoz-Galdeano T, Reigada D, Del Águila Á, Velez I, Caballero-López MJ, Maza RM, Nieto-Díaz M
Frontiers in cellular neuroscience 2018;12:164
Frontiers in cellular neuroscience 2018;12:164
Oleuropein attenuates hydrogen peroxide-induced autophagic cell death in human adipose-derived stem cells.
Ji ST, Kim YJ, Jung SY, Kim DY, Kang S, Park JH, Jang WB, Ha J, Yun J, Kwon SM
Biochemical and biophysical research communications 2018 May 15;499(3):675-680
Biochemical and biophysical research communications 2018 May 15;499(3):675-680
ULK1 O-GlcNAcylation Is Crucial for Activating VPS34 via ATG14L during Autophagy Initiation.
Pyo KE, Kim CR, Lee M, Kim JS, Kim KI, Baek SH
Cell reports 2018 Dec 4;25(10):2878-2890.e4
Cell reports 2018 Dec 4;25(10):2878-2890.e4
MicroRNA-101 Modulates Autophagy and Oligodendroglial Alpha-Synuclein Accumulation in Multiple System Atrophy.
Valera E, Spencer B, Mott J, Trejo M, Adame A, Mante M, Rockenstein E, Troncoso JC, Beach TG, Masliah E, Desplats P
Frontiers in molecular neuroscience 2017;10:329
Frontiers in molecular neuroscience 2017;10:329
Negative LC3b immunoreactivity in cancer cells is an independent prognostic predictor of prostate cancer specific death.
Mortezavi A, Salemi S, Rupp NJ, Rüschoff JH, Hermanns T, Poyet C, Randazzo M, Simon HU, Moch H, Sulser T, Wild P, Eberli D
Oncotarget 2017 May 9;8(19):31765-31774
Oncotarget 2017 May 9;8(19):31765-31774
IRE1α promotes viral infection by conferring resistance to apoptosis.
Fink SL, Jayewickreme TR, Molony RD, Iwawaki T, Landis CS, Lindenbach BD, Iwasaki A
Science signaling 2017 Jun 6;10(482)
Science signaling 2017 Jun 6;10(482)
Ginsenoside Rg1 protects against sepsis-associated encephalopathy through beclin 1-independent autophagy in mice.
Li Y, Wang F, Luo Y
The Journal of surgical research 2017 Jan;207:181-189
The Journal of surgical research 2017 Jan;207:181-189
Human Rad23A plays a regulatory role in autophagy.
Tan X, Wang HC, Liang RY, Chuang SM
Biochemical and biophysical research communications 2016 Sep 30;478(4):1772-9
Biochemical and biophysical research communications 2016 Sep 30;478(4):1772-9
Absence of autophagy promotes apoptosis by modulating the ROS-dependent RLR signaling pathway in classical swine fever virus-infected cells.
Pei J, Deng J, Ye Z, Wang J, Gou H, Liu W, Zhao M, Liao M, Yi L, Chen J
Autophagy 2016 Oct 2;12(10):1738-1758
Autophagy 2016 Oct 2;12(10):1738-1758
Amber Light (590 nm) Induces the Breakdown of Lipid Droplets through Autophagy-Related Lysosomal Degradation in Differentiated Adipocytes.
Choi MS, Kim HJ, Ham M, Choi DH, Lee TR, Shin DW
Scientific reports 2016 Jun 27;6:28476
Scientific reports 2016 Jun 27;6:28476
Hinokitiol induces autophagy in murine breast and colorectal cancer cells.
Wang WK, Lin ST, Chang WW, Liu LW, Li TY, Kuo CY, Hsieh JL, Lee CH
Environmental toxicology 2016 Jan;31(1):77-84
Environmental toxicology 2016 Jan;31(1):77-84
Autophagy Inhibition Delays Early but Not Late-Stage Metastatic Disease.
Barnard RA, Regan DP, Hansen RJ, Maycotte P, Thorburn A, Gustafson DL
The Journal of pharmacology and experimental therapeutics 2016 Aug;358(2):282-93
The Journal of pharmacology and experimental therapeutics 2016 Aug;358(2):282-93
A novel curcumin analog binds to and activates TFEB in vitro and in vivo independent of MTOR inhibition.
Song JX, Sun YR, Peluso I, Zeng Y, Yu X, Lu JH, Xu Z, Wang MZ, Liu LF, Huang YY, Chen LL, Durairajan SS, Zhang HJ, Zhou B, Zhang HQ, Lu A, Ballabio A, Medina DL, Guo Z, Li M
Autophagy 2016 Aug 2;12(8):1372-89
Autophagy 2016 Aug 2;12(8):1372-89
Interference of Notch1 inhibits the growth of glioma cancer cells by inducing cell autophagy and down-regulation of Notch1-Hes-1 signaling pathway.
Yao J, Zheng K, Li C, Liu H, Shan X
Medical oncology (Northwood, London, England) 2015 Jun;32(6):610
Medical oncology (Northwood, London, England) 2015 Jun;32(6):610
In Vitro and in Vivo Atheroprotective Effects of Gossypetin against Endothelial Cell Injury by Induction of Autophagy.
Lin HH
Chemical research in toxicology 2015 Feb 16;28(2):202-15
Chemical research in toxicology 2015 Feb 16;28(2):202-15
Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine.
Lin CW, Jan MS, Kuo JH
Molecular pharmaceutics 2015 Aug 3;12(8):2858-68
Molecular pharmaceutics 2015 Aug 3;12(8):2858-68
Sirtuin 1 (SIRT1) Deacetylase Activity and NAD⁺/NADH Ratio Are Imperative for Capsaicin-Mediated Programmed Cell Death.
Lee YH, Chen HY, Su LJ, Chueh PJ
Journal of agricultural and food chemistry 2015 Aug 26;63(33):7361-70
Journal of agricultural and food chemistry 2015 Aug 26;63(33):7361-70
Autophagy-related gene expression analysis of wild-type and atg5 gene knockout mouse embryonic fibroblast cells treated with polyethylenimine.
Lin CW, Jan MS, Kuo JH
Molecular pharmaceutics 2014 Sep 2;11(9):3002-8
Molecular pharmaceutics 2014 Sep 2;11(9):3002-8
Autophagy enhances the replication of classical swine fever virus in vitro.
Pei J, Zhao M, Ye Z, Gou H, Wang J, Yi L, Dong X, Liu W, Luo Y, Liao M, Chen J
Autophagy 2014 Jan;10(1):93-110
Autophagy 2014 Jan;10(1):93-110
HMGB1 is involved in autophagy inhibition caused by SNCA/α-synuclein overexpression: a process modulated by the natural autophagy inducer corynoxine B.
Song JX, Lu JH, Liu LF, Chen LL, Durairajan SS, Yue Z, Zhang HQ, Li M
Autophagy 2014 Jan;10(1):144-54
Autophagy 2014 Jan;10(1):144-54
Maintenance of basal levels of autophagy in Huntington's disease mouse models displaying metabolic dysfunction.
Baldo B, Soylu R, Petersén A
PloS one 2013;8(12):e83050
PloS one 2013;8(12):e83050
BECN1/Beclin 1 is recruited into lipid rafts by prion to activate autophagy in response to amyloid β 42.
Nah J, Pyo JO, Jung S, Yoo SM, Kam TI, Chang J, Han J, Soo A An S, Onodera T, Jung YK
Autophagy 2013 Dec;9(12):2009-21
Autophagy 2013 Dec;9(12):2009-21
BECN1/Beclin 1 is recruited into lipid rafts by prion to activate autophagy in response to amyloid β 42.
Nah J, Pyo JO, Jung S, Yoo SM, Kam TI, Chang J, Han J, Soo A An S, Onodera T, Jung YK
Autophagy 2013 Dec;9(12):2009-21
Autophagy 2013 Dec;9(12):2009-21
Influenza A virus proteins NS1 and hemagglutinin along with M2 are involved in stimulation of autophagy in infected cells.
Zhirnov OP, Klenk HD
Journal of virology 2013 Dec;87(24):13107-14
Journal of virology 2013 Dec;87(24):13107-14
Autophagy proteins stabilize pathogen-containing phagosomes for prolonged MHC II antigen processing.
Romao S, Gasser N, Becker AC, Guhl B, Bajagic M, Vanoaica D, Ziegler U, Roesler J, Dengjel J, Reichenbach J, Münz C
The Journal of cell biology 2013 Dec 9;203(5):757-66
The Journal of cell biology 2013 Dec 9;203(5):757-66
8-Amino-adenosine activates p53-independent cell death of metastatic breast cancers.
Polotskaia A, Hoffman S, Krett NL, Shanmugam M, Rosen ST, Bargonetti J
Molecular cancer therapeutics 2012 Nov;11(11):2495-504
Molecular cancer therapeutics 2012 Nov;11(11):2495-504
PML-RARα enhances constitutive autophagic activity through inhibiting the Akt/mTOR pathway.
Huang Y, Hou JK, Chen TT, Zhao XY, Yan ZW, Zhang J, Yang J, Kogan SC, Chen GQ
Autophagy 2011 Oct;7(10):1132-44
Autophagy 2011 Oct;7(10):1132-44
Presenilin is necessary for efficient proteolysis through the autophagy-lysosome system in a γ-secretase-independent manner.
Neely KM, Green KN, LaFerla FM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Feb 23;31(8):2781-91
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Feb 23;31(8):2781-91
Profound cardioprotection with chloramphenicol succinate in the swine model of myocardial ischemia-reperfusion injury.
Sala-Mercado JA, Wider J, Undyala VV, Jahania S, Yoo W, Mentzer RM Jr, Gottlieb RA, Przyklenk K
Circulation 2010 Sep 14;122(11 Suppl):S179-84
Circulation 2010 Sep 14;122(11 Suppl):S179-84
Hypoxia-induced autophagy is mediated through hypoxia-inducible factor induction of BNIP3 and BNIP3L via their BH3 domains.
Bellot G, Garcia-Medina R, Gounon P, Chiche J, Roux D, Pouysségur J, Mazure NM
Molecular and cellular biology 2009 May;29(10):2570-81
Molecular and cellular biology 2009 May;29(10):2570-81
Effect of autophagy on multiple myeloma cell viability.
Hoang B, Benavides A, Shi Y, Frost P, Lichtenstein A
Molecular cancer therapeutics 2009 Jul;8(7):1974-84
Molecular cancer therapeutics 2009 Jul;8(7):1974-84
Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages.
Kyei GB, Dinkins C, Davis AS, Roberts E, Singh SB, Dong C, Wu L, Kominami E, Ueno T, Yamamoto A, Federico M, Panganiban A, Vergne I, Deretic V
The Journal of cell biology 2009 Jul 27;186(2):255-68
The Journal of cell biology 2009 Jul 27;186(2):255-68
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Beclin 1 Antibody [NB110-87318] - Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Beclin 1 Antibody [NB110-87318] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Beclin 1 Antibody [NB110-87318] - Lane view shows a specific band for Beclin in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Beclin 1 Antibody [NB110-87318] - Beclin 1/ATG6 Antibody [NB110-87318] - Detection of Beclin 1 in HeLa whole cell lysate at 1:10,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Beclin 1 Antibody [NB110-87318] - Basal levels of autophagy in the hypothalamus of BACHD mice. The expression levels of selected autophagy markers were analyzed by Western blot in hypothalamic tissue from BACHD mice at 2, 6 and 12 months of age and compared with gender- and age-matched controls (n=3-6/group). The data showed a substantial maintenance of the autophagy flux, despite the presence of a metabolic phenotype in the BACHD mice already at early time points. The data in the graphs are expressed as mean +/- SEM, relative to the 2 months wt controls. The Western blots are representative of one sample for each group. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0083050), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of normal breast tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Beclin 1/ATG6 Antibody [NB110-87318] - Staining of breast carcinoma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot 71917) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Beclin 1 Antibody [NB110-87318] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (Lot F4) at 1:300 dilution. The primary antibody bound to Beclin 1 antigen in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. Cytoplasmic staining can be seen in the cancer as well as the stromal cells. The signal was strongest in a subset of stromal cells which appears to be the cancer associated fibroblasts.
