Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-20171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Beclin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A431 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Poliovirus induces autophagic signaling independent of the ULK1 complex.
Corona Velazquez A, Corona AK, Klein KA, Jackson WT
Autophagy 2018;14(7):1201-1213
Autophagy 2018;14(7):1201-1213
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A431 cell lysate using a Beclin-1 polyclonal antibody (Product # PA5-20171) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Beclin-1 in A431 cell lysate with Beclin 1 Polyclonal Antibody (Product # PA5-20171) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using a Beclin-1 polyclonal antibody (Product # PA5-20171) at a 2 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Beclin-1 (green) HEK293T cells. Cells fixed with 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beclin-1 polyclonal antibody (Product # PA5-20171) at a dilution of 1:100 overnight at 4°C in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken on a Leica DM1000 microscope at 40X magnification. Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of A431 cells using Beclin 1 Polyclonal Antibody (Product # PA5-20171) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Beclin-1 in A431 cells with Beclin 1 Polyclonal Antibody (Product # PA5-20171) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of A431 cells using a Beclin-1 polyclonal antibody (Product # PA5-20171) at a 1 µg/mL dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
