Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- EB08365 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB08365, RRID:AB_2154213
- Product name
- Goat anti-NRAS Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-NRAS Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-RMKKLNSSDDGTQ
- Epitope
- internal region
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- HEK293 overexpressing NRAS (RC202681) and probed with EB08365 (mock transfection in first lane), tested by Origene.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08365 (0.01µg/ml) staining of A431 lysate (35µg protein in RIPA buffer).Primary incubation was 1 hour.Detected by chemiluminescence.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08365 (0.1µg/ml) staining of Rat Intestines lysate (35µg protein in RIPA buffer).Primary incubation was 1 hour.Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08365 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.