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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6028 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TTK Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TTK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CaM Kinase II, rhCAM Kinase II alpha , rhCaM Kinase II beta , rhCaM Kinase II delta , rhCaM Kinase II gamma , rhCaM Kinase III, rhCaM Kinase IV, rhCaM Kinase K alpha , or rhCaM Kinase K beta is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P33981- Isotype
- IgG
- Antibody clone number
- 689913
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human TTK by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TTK Monoclonal Antibody (Catalog # MAB6028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TTK at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
