AMAb91117

antibody from Atlas Antibodies

Targeting: LAMA1 LAMA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb91117

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91117, RRID:AB_2665807

Product name

Anti-LAMA1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

TVSYDIPVETVDSNLMSHADVIIKGNGLTLSTQAE
GLSLQPYEEYLNVVRLVPENFQDFHSKRQIDRDQL
MTVLANVTHLLIRANYNSAKMALYRLESVS


Epitope

Binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL3087

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-221, Laminin-332, Laminin-411 and Laminin-521.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human testis tissue.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and lymph node tissues using AMAb91117 antibody. Corresponding LAMA1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate immunoreactivity in basement membrane of seminiferous tubules.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows absence of immunoreactivity in lymphoid tissue (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate positivity in basement membrane of cells in seminiferous ducts.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows no positivity in lymphoid cells as expected.