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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-16287 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Laminin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA5-16291 targets Glial Fibrillary Acidic Protein in IHC (P) applications and shows reactivity with Bovine, Chicken, Guinea Pig, Hamster, Human, mouse, Non-human primate, Ovine, and Rat samples. The PA5-16291 immunogen is gFAP isolated from cow spinal cord.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C
Submitted references Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy.
Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration.
Masticatory muscles of mouse do not undergo atrophy in space.
Two waves of anisotropic growth generate enlarged follicles in the spiny mouse.
Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.
Phenotypic characteristics and proliferative activity of hyperplastic ductule cells in cholangiofibrosis induced by thioacetamide in rats.
Rescue of dystrophic skeletal muscle by PGC-1α involves restored expression of dystrophin-associated protein complex components and satellite cell signaling.
Regenerative responses in slow- and fast-twitch muscles following moderate contusion spinal cord injury and locomotor training.
Hindlimb muscle morphology and function in a new atrophy model combining spinal cord injury and cast immobilization.
Immunocytochemistry to study myogenesis in zebrafish.
The nanomechanical signature of breast cancer.
Topographic characterization and protein quantification of esophageal basement membrane for scaffold design reference in tissue engineering.
The effect of valproic acid and oxcarbazepine on the distribution of adhesion molecules in embryo implantation.
Woodall BP, Woodall MC, Luongo TS, Grisanti LA, Tilley DG, Elrod JW, Koch WJ
The Journal of biological chemistry 2016 Oct 14;291(42):21913-21924
The Journal of biological chemistry 2016 Oct 14;291(42):21913-21924
Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration.
Govindan J, Iovine MK
Gene expression patterns : GEP 2015 Sep-Nov;19(1-2):21-9
Gene expression patterns : GEP 2015 Sep-Nov;19(1-2):21-9
Masticatory muscles of mouse do not undergo atrophy in space.
Philippou A, Minozzo FC, Spinazzola JM, Smith LR, Lei H, Rassier DE, Barton ER
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Jul;29(7):2769-79
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Jul;29(7):2769-79
Two waves of anisotropic growth generate enlarged follicles in the spiny mouse.
Montandon SA, Tzika AC, Martins AF, Chopard B, Milinkovitch MC
EvoDevo 2014;5:33
EvoDevo 2014;5:33
Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions.
Subramanian A, Schilling TF
eLife 2014 Jun 18;3
eLife 2014 Jun 18;3
Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
Brisson BK, Spinazzola J, Park S, Barton ER
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
American journal of physiology. Endocrinology and metabolism 2014 Apr 15;306(8):E965-74
Label retaining cells (LRCs) with myoepithelial characteristic from the proximal acinar region define stem cells in the sweat gland.
Leung Y, Kandyba E, Chen YB, Ruffins S, Kobielak K
PloS one 2013;8(9):e74174
PloS one 2013;8(9):e74174
Phenotypic characteristics and proliferative activity of hyperplastic ductule cells in cholangiofibrosis induced by thioacetamide in rats.
Hata M, Iida H, Yamanegi K, Yamada N, Ohyama H, Hirano H, Nakasho K, Terada N
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2013 Mar;65(3):351-6
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie 2013 Mar;65(3):351-6
Rescue of dystrophic skeletal muscle by PGC-1α involves restored expression of dystrophin-associated protein complex components and satellite cell signaling.
Hollinger K, Gardan-Salmon D, Santana C, Rice D, Snella E, Selsby JT
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Jul 1;305(1):R13-23
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Jul 1;305(1):R13-23
Regenerative responses in slow- and fast-twitch muscles following moderate contusion spinal cord injury and locomotor training.
Jayaraman A, Liu M, Ye F, Walter GA, Vandenborne K
European journal of applied physiology 2013 Jan;113(1):191-200
European journal of applied physiology 2013 Jan;113(1):191-200
Hindlimb muscle morphology and function in a new atrophy model combining spinal cord injury and cast immobilization.
Ye F, Baligand C, Keener JE, Vohra R, Lim W, Ruhella A, Bose P, Daniels M, Walter GA, Thompson F, Vandenborne K
Journal of neurotrauma 2013 Feb 1;30(3):227-35
Journal of neurotrauma 2013 Feb 1;30(3):227-35
Immunocytochemistry to study myogenesis in zebrafish.
Bird NC, Windner SE, Devoto SH
Methods in molecular biology (Clifton, N.J.) 2012;798:153-69
Methods in molecular biology (Clifton, N.J.) 2012;798:153-69
The nanomechanical signature of breast cancer.
Plodinec M, Loparic M, Monnier CA, Obermann EC, Zanetti-Dallenbach R, Oertle P, Hyotyla JT, Aebi U, Bentires-Alj M, Lim RY, Schoenenberger CA
Nature nanotechnology 2012 Nov;7(11):757-65
Nature nanotechnology 2012 Nov;7(11):757-65
Topographic characterization and protein quantification of esophageal basement membrane for scaffold design reference in tissue engineering.
Li Y, Zhu Y, Yu H, Chen L, Liu Y
Journal of biomedical materials research. Part B, Applied biomaterials 2012 Jan;100(1):265-73
Journal of biomedical materials research. Part B, Applied biomaterials 2012 Jan;100(1):265-73
The effect of valproic acid and oxcarbazepine on the distribution of adhesion molecules in embryo implantation.
Gürgen SG, Erdoğan D, Coşkun ZK, Cansu A
Toxicology 2012 Feb 26;292(2-3):71-7
Toxicology 2012 Feb 26;292(2-3):71-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Laminin was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Laminin Rabbit Polyclonal Antibody (Product # PA5-16287) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing extracellular matrix localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human skin stained with Laminin antibody using peroxidase-conjugate and AEC chromogen. Note staining of basement membranes of epidermis and surrounding blood vessels.
