Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-79802 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79802, RRID:AB_1108371
- Product name
- Rabbit Polyclonal CRM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Enhanced nuclear protein export in premature aging and rescue of the progeria phenotype by modulation of CRM1 activity.
Expression and Intracellular Localization of Paraoxonase 2 in Different Types of Malignancies.
Control of nuclear β-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function.
The Second-Generation Exportin-1 Inhibitor KPT-8602 Demonstrates Potent Activity against Acute Lymphoblastic Leukemia.
Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes.
The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.
Casein-kinase-II-dependent phosphorylation of PPARgamma provokes CRM1-mediated shuttling of PPARgamma from the nucleus to the cytosol.
García-Aguirre I, Alamillo-Iniesta A, Rodríguez-Pérez R, Vélez-Aguilera G, Amaro-Encarnación E, Jiménez-Gutiérrez E, Vásquez-Limeta A, Samuel Laredo-Cisneros M, Morales-Lázaro SL, Tiburcio-Félix R, Ortega A, Magaña JJ, Winder SJ, Cisneros B
Aging cell 2019 Oct;18(5):e13002
Aging cell 2019 Oct;18(5):e13002
Expression and Intracellular Localization of Paraoxonase 2 in Different Types of Malignancies.
Shakhparonov MI, Antipova NV, Shender VO, Shnaider PV, Arapidi GP, Pestov NB, Pavlyukov MS
Acta naturae 2018 Jul-Sep;10(3):92-99
Acta naturae 2018 Jul-Sep;10(3):92-99
Control of nuclear β-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function.
Vélez-Aguilera G, de Dios Gómez-López J, Jiménez-Gutiérrez GE, Vásquez-Limeta A, Laredo-Cisneros MS, Gómez P, Winder SJ, Cisneros B
Biochimica et biophysica acta. Molecular cell research 2018 Feb;1865(2):406-420
Biochimica et biophysica acta. Molecular cell research 2018 Feb;1865(2):406-420
The Second-Generation Exportin-1 Inhibitor KPT-8602 Demonstrates Potent Activity against Acute Lymphoblastic Leukemia.
Vercruysse T, De Bie J, Neggers JE, Jacquemyn M, Vanstreels E, Schmid-Burgk JL, Hornung V, Baloglu E, Landesman Y, Senapedis W, Shacham S, Dagklis A, Cools J, Daelemans D
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 May 15;23(10):2528-2541
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 May 15;23(10):2528-2541
Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes.
Oka M, Mura S, Yamada K, Sangel P, Hirata S, Maehara K, Kawakami K, Tachibana T, Ohkawa Y, Kimura H, Yoneda Y
eLife 2016 Jan 7;5:e09540
eLife 2016 Jan 7;5:e09540
The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.
Oka M, Asally M, Yasuda Y, Ogawa Y, Tachibana T, Yoneda Y
Molecular biology of the cell 2010 Jun 1;21(11):1885-96
Molecular biology of the cell 2010 Jun 1;21(11):1885-96
Casein-kinase-II-dependent phosphorylation of PPARgamma provokes CRM1-mediated shuttling of PPARgamma from the nucleus to the cytosol.
von Knethen A, Tzieply N, Jennewein C, Brüne B
Journal of cell science 2010 Jan 15;123(Pt 2):192-201
Journal of cell science 2010 Jan 15;123(Pt 2):192-201
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
