Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF971 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse TIMP-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse TIMP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human TIMP-4 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16035
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bone-derived Nestin-positive mesenchymal stem cells improve cardiac function via recruiting cardiac endothelial cells after myocardial infarction.
Human umbilical cord plasma proteins revitalize hippocampal function in aged mice.
Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcome.
Inhibition of airway epithelial-to-mesenchymal transition and fibrosis by kaempferol in endotoxin-induced epithelial cells and ovalbumin-sensitized mice.
Fibroblast-conditioned media promote human sarcoma cell invasion.
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Lu D, Liao Y, Zhu SH, Chen QC, Xie DM, Liao JJ, Feng X, Jiang MH, He W
Stem cell research & therapy 2019 Apr 27;10(1):127
Stem cell research & therapy 2019 Apr 27;10(1):127
Human umbilical cord plasma proteins revitalize hippocampal function in aged mice.
Castellano JM, Mosher KI, Abbey RJ, McBride AA, James ML, Berdnik D, Shen JC, Zou B, Xie XS, Tingle M, Hinkson IV, Angst MS, Wyss-Coray T
Nature 2017 Apr 27;544(7651):488-492
Nature 2017 Apr 27;544(7651):488-492
Mitral tissue inhibitor of metalloproteinase 2 is associated with mitral valve surgery outcome.
Lin TH, Yang SF, Chiu CC, Su HM, Voon WC, Chai CY, Lai WT, Sheu SH
PloS one 2014;9(1):e86287
PloS one 2014;9(1):e86287
Inhibition of airway epithelial-to-mesenchymal transition and fibrosis by kaempferol in endotoxin-induced epithelial cells and ovalbumin-sensitized mice.
Gong JH, Cho IH, Shin D, Han SY, Park SH, Kang YH
Laboratory investigation; a journal of technical methods and pathology 2014 Mar;94(3):297-308
Laboratory investigation; a journal of technical methods and pathology 2014 Mar;94(3):297-308
Fibroblast-conditioned media promote human sarcoma cell invasion.
Bittner JG 4th, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
Surgery 2009 Jan;145(1):42-7
Surgery 2009 Jan;145(1):42-7
Coregulation of vascular tube stabilization by endothelial cell TIMP-2 and pericyte TIMP-3.
Saunders WB, Bohnsack BL, Faske JB, Anthis NJ, Bayless KJ, Hirschi KK, Davis GE
The Journal of cell biology 2006 Oct 9;175(1):179-91
The Journal of cell biology 2006 Oct 9;175(1):179-91
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TIMP-2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TIMP-2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Neutralization of TIMP-2 Activity by Human TIMP-2 Antibody. Recombinant Human MMP-2 (0.2 µg/mL, Catalog # 902-MPN or 902-MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971). The ND50 is typically 2.6 µg/mL.