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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB971 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIMP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human TIMP-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIMP-1 or recombinant mouse TIMP-1 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P16035- Isotype
- IgG
- Antibody clone number
- 89025
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MicroRNA‑122 acts as tumor suppressor by targeting TRIM29 and blocking the activity of PI3K/AKT signaling in nasopharyngeal carcinoma in vitro.
Granule exocytosis is required for platelet spreading: differential sorting of α-granules expressing VAMP-7.
Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy.
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Matrix metalloproteinase activity in pediatric acute lung injury.
Matrix metalloproteinase activity in pediatric acute lung injury.
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Yang Y, Li Q, Guo L
Molecular medicine reports 2018 Jun;17(6):8244-8252
Molecular medicine reports 2018 Jun;17(6):8244-8252
Granule exocytosis is required for platelet spreading: differential sorting of α-granules expressing VAMP-7.
Peters CG, Michelson AD, Flaumenhaft R
Blood 2012 Jul 5;120(1):199-206
Blood 2012 Jul 5;120(1):199-206
Conditionally replicating adenovirus expressing TIMP2 for ovarian cancer therapy.
Yang SW, Cody JJ, Rivera AA, Waehler R, Wang M, Kimball KJ, Alvarez RA, Siegal GP, Douglas JT, Ponnazhagan S
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Feb 1;17(3):538-49
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Feb 1;17(3):538-49
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biology of reproduction 2010 May;82(5):968-79
Biology of reproduction 2010 May;82(5):968-79
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Experimental hematology 2009 Jul;37(7):849-56
Experimental hematology 2009 Jul;37(7):849-56
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut 2006 Dec;55(12):1774-80
Gut 2006 Dec;55(12):1774-80
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human TIMP-2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TIMP-2 at approximately 22 kDa (as indicated).This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TIMP-2 in Human Ovarian Cancer Tissue. TIMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Mouse Anti-Human TIMP-2 Monoclonal Antibody (Catalog # MAB971) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
