H00027032-M01

antibody from Novus Biologicals

Targeting: ATP2C1 ATP2C1A, BCPM, KIAA1347, PMR1, SPCA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00027032-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00027032-M01, RRID:AB_537005

Product name

Mouse Monoclonal ATP2C1 Antibody

Antibody type

Monoclonal

Description

IgG purified. ATP2C1 - ATPase, Ca++ transporting, type 2C, member 1

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network.

Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J
Developmental cell 2018 Nov 19;47(4):464-478.e8

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Diverse Viruses Require the Calcium Transporter SPCA1 for Maturation and Spread.

Hoffmann HH, Schneider WM, Blomen VA, Scull MA, Hovnanian A, Brummelkamp TR, Rice CM
Cell host & microbe 2017 Oct 11;22(4):460-470.e5

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Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting.

Kienzle C, Basnet N, Crevenna AH, Beck G, Habermann B, Mizuno N, von Blume J
The Journal of cell biology 2014 Sep 1;206(5):635-54

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Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231.

Grice DM, Vetter I, Faddy HM, Kenny PA, Roberts-Thomson SJ, Monteith GR
The Journal of biological chemistry 2010 Nov 26;285(48):37458-66

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi ( Cat # H00027032-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATP2C1 monoclonal antibody (M01), clone 2G1 (Cat # H00027032-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody (M01), clone 2G1.Lane 1: ATP2C1 transfected lysate(100.6 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ATP2C1 Antibody (2G1) [H00027032-M01] - ATP2C1 monoclonal antibody (M01), clone 2G1 Analysis of ATP2C1 expression in HeLa.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: ATP2C1 Antibody (2G1) [H00027032-M01] - Detection limit for recombinant GST tagged ATP2C1 is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of monoclonal antibody to ATP2C1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ATP2C1 Antibody (2G1) [H00027032-M01] - Analysis of ATP2C1 transfected lysate using anti-ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 MaxPab rabbit polyclonal antibody.