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LearnH00027032-M01
antibody from Invitrogen Antibodies
Targeting: ATP2C1
ATP2C1A, BCPM, KIAA1347, PMR1, SPCA1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [2]
- Immunocytochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- H00027032-M01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP2C1 Monoclonal Antibody (2G1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Sequence of this protein is as follows: VQEYRSEKSL EELSKLVPPE CHCVREGKLE HTLARDLVPG DTVCLSVGDR VPADLRLFEA VDLSIDESSL TGETTPCSKV TAPQPAATNG DLASRSNIAF MGTLVRCGKA KGVVIGTGEN SEFGEVFKMM QAEEAPKTPL QKSMDLLGKQ L
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G1
- Vial size
- 100 µg
- Concentration
- See Label
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody (M01), clone 2G1.Lane 1: ATP2C1 transfected lysate(100.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi (Cat # H00027032-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ATP2C1 monoclonal antibody (M01), clone 2G1 (Cat # H00027032-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody (M01), clone 2G1.Lane 1: ATP2C1 transfected lysate(100.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATP2C1 monoclonal antibody (M01), clone 2G1 Western Blot analysis of ATP2C1 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ATP2C1 is approximately 0.03 ng/mL as a capture antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ATP2C1 is approximately 0.03 ng/mL as a capture antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ATP2C1 on HeLa cell. Antibody concentration is 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATP2C1 transfected lysate using anti-ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 MaxPab rabbit polyclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATP2C1 transfected lysate using anti-ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 MaxPab rabbit polyclonal antibody.
