Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-97870 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD127 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 5 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Human Lung-Resident Macrophages Express and Are Targets of Thymic Stromal Lymphopoietin in the Tumor Microenvironment.
Braile M, Fiorelli A, Sorriento D, Di Crescenzo RM, Galdiero MR, Marone G, Santini M, Varricchi G, Loffredo S
Cells 2021 Aug 6;10(8)
Cells 2021 Aug 6;10(8)
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of CD127 using a CD127 Polyclonal antibody (Product # PA5-97870) at a concentration of 4 µg/mL. Lane 1: K562 whole cell lysate. Lane 2: Jurkat whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 52 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD127 in paraffin embedded human colon cancer using a CD127 polyclonal antibody (Product # PA5-97870) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of CD127 in paraffin embedded human skin tissue using a CD127 polyclonal antibody (Product # PA5-97870) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 1 Constitutive expression of TSLP system in human lung macrophages (HLMs). The constitutive expression of sfTSLP, lfTSLP, TSLPR and IL-7Ralpha mRNAs was evaluated by quantitative RT-PCR in highly purified HLMs (4.5 x 10 6 cells/well) ( A ). Data are mean +- SD of 6 independent experiments obtained from different patients. Cytocentrifuge preparations of HLMs were immunohistochemically stained for TSLP ( C ), TSLPR ( D ), and IL-7Ralpha ( E ) with specific primary antibodies or in absence ( B ) of primary antibody (CTRL) as described in Materials and Methods. Microscope magnification 60x. Results are representative of 6 independent experiments obtained from different patients. * p < 0.01 when compared to lfTSLP, TSLPR, IL-7Ralpha. px: pixels.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 7 Expression of TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer by immunohistochemistry. Immunohistochemical staining for TSLP, TSLPR, and IL-7Ralpha in peritumoral and intratumoral human lung cancer. In the control (CTRL), the primary antibody was omitted. Microscope magnification 20x. This experiment is representative of 5 experiments obtained from different patients.