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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF2956 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse MARCO Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse MARCO in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q60754- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Aging-Impaired Filamentous Actin Polymerization Signaling Reduces Alveolar Macrophage Phagocytosis of Bacteria.
Role of lysosomes in silica-induced inflammasome activation and inflammation in absence of MARCO.
Identification of an IFN-γ/mast cell axis in a mouse model of chronic asthma.
Li Z, Jiao Y, Fan EK, Scott MJ, Li Y, Li S, Billiar TR, Wilson MA, Shi X, Fan J
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3176-3186
Journal of immunology (Baltimore, Md. : 1950) 2017 Nov 1;199(9):3176-3186
Role of lysosomes in silica-induced inflammasome activation and inflammation in absence of MARCO.
Biswas R, Hamilton RF Jr, Holian A
Journal of immunology research 2014;2014:304180
Journal of immunology research 2014;2014:304180
Identification of an IFN-γ/mast cell axis in a mouse model of chronic asthma.
Yu M, Eckart MR, Morgan AA, Mukai K, Butte AJ, Tsai M, Galli SJ
The Journal of clinical investigation 2011 Aug;121(8):3133-43
The Journal of clinical investigation 2011 Aug;121(8):3133-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Mouse MARCO by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MARCO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2956) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for MARCO at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
