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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-68302 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with Mouse and Rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Vesicular and uncoated Rab1-dependent cargo carriers facilitate ER to Golgi transport.
Westrate LM, Hoyer MJ, Nash MJ, Voeltz GK
Journal of cell science 2020 Jul 24;133(14)
Journal of cell science 2020 Jul 24;133(14)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAB1B in COLO205 whole cell lysates using a RAB1B Polyclonal Antibody (Product # PA5-68302).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RAB1B in paraffin-embedded human colon cancer. 1) RAB1B Polyclonal Antibody (Product # PA5-68302) was diluted at 1:200 (4° overnight). 2) Tris-EDTA,pH9.0 was used for antigen retrieval. 3) Secondary antibody was diluted at 1:200 (room temperature, 45 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7. Rab1 labels uncoated cargo carriers. (A,B) Representative examples of a COS-7 cell expressing mCh-TNF RUSH (red), GFP-Sec24D (gray) and BFP-Rab1b (green) before (A) or after (B) biotin addition. In A, before biotin addition, the Sec24D, TNF cargo and Rab1b all accumulate in the same place. In B, after biotin is added, Rab1b and cargo localize to structures not marked by Sec24D. (C) Time-lapse image series of an event from B where fluorescently marked cargo (red) and Rab1b (green) are observed leaving the Sec24D-labeled ERES (gray). The original site is marked with a yellow arrow in each frame and the leaving cargo vesicle is marked with a white arrow. (D) For the event in C, BFP-Rab1b, mCh-TNF RUSH cargo and GFP-Sec24D fluorescence intensity was monitored throughout the 2 min movie at the ERES, as indicated by the dotted circle region. Sec24D fluorescence levels do not drop when the cargo leaves. TNF cargo fluorescence and Rab1b fluorescence both drop when cargo leaves (frame defined by the arrow). (E) Quantification of Rab1b, TNF cargo and Sec24D fluorescence immediately before (pre) and immediately after (post) cargo leaves the ERES for multiple export events (16 events from 9 cells). Circles mark where fluorescence measurements were made: ROI1 is the site where cargo leaves from and ROI2 is the site where cargo traffics to. Fluorescence at each region for each marker was background subtracted and the percentage fluorescence at each ROI was calculated pre and post c
