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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM08439PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM08439PU-N, RRID:AB_2035863
- Product name
- anti PMS2 (58-81)
- Antibody type
- Monoclonal
- Antigen
- Recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 349.29.5.2
- Vial size
- 0.1 mg
- Concentration
- 1.38 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot analysis is shown using Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 μg of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignalTM substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.
