Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-59159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SP3/SP4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: NQATGQQQII IDPSQGLVQL QNQPQQLELV TTQLAGNAWQ LVASTPPASK ENNVSQP
- Concentration
- 0.1 mg/mL
Submitted references Oestrogen-related receptor alpha mediates chemotherapy resistance of osteosarcoma cells via regulation of ABCB1.
Chen Y, Zhang K, Li Y, Guo R, Zhang K, Zhong G, He Q
Journal of cellular and molecular medicine 2019 Mar;23(3):2115-2124
Journal of cellular and molecular medicine 2019 Mar;23(3):2115-2124
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP3/SP4 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a SP3/SP4 Polyclonal Antibody (Product # PA5-59159).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SP3/SP4 in human cell line U-251 MG using a SP3/SP4 Polyclonal Antibody (Product # PA5-59159) shows localization to nucleoplasm and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SP3/SP4 in human lymph node shows distinct nuclear positivity in non-germinal center cells. Samples were probed using a SP3/SP4 Polyclonal Antibody (Product # PA5-59159).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Oestrogen-related receptors alpha ( ERR alpha) bound to SP 3 to increase the transcription of ABCB 1. The ERR alpha was immunoprecipitated by use of its specific antibody in MG -63/Dox (A) or HOS /Dox (B) and their parental cells, respectively. The bound SP 3 was measured by western blot analysis, IgG was used as the negative control; (C) MG -63/Dox cells were transfected with si- NC or si- SP 3 for 24 h; MG -63/Dox cells were transfected with si- NC or si- SP 3 for 12 h and then further treated with or without 1 mumol/L XCT -790 for 24 h, the mRNA (D) or protein (E) expression of P-gp was measured and quantitatively analyzed. Data are presented as means +- SD of three independent experiments. ** P < 0.01 compared with control
