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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-676 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-676, RRID:AB_673504
- Product name
- Anti-CSF2RB
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Interleukin-5 receptor subunit oligomerization and rearrangement revealed by fluorescence resonance energy transfer imaging.
A unique element in the cytoplasmic tail of the type II transforming growth factor-beta receptor controls basolateral delivery.
Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
Zaks-Zilberman M, Harrington AE, Ishino T, Chaiken IM
The Journal of biological chemistry 2008 May 9;283(19):13398-406
The Journal of biological chemistry 2008 May 9;283(19):13398-406
A unique element in the cytoplasmic tail of the type II transforming growth factor-beta receptor controls basolateral delivery.
Murphy SJ, Shapira KE, Henis YI, Leof EB
Molecular biology of the cell 2007 Oct;18(10):3788-99
Molecular biology of the cell 2007 Oct;18(10):3788-99
Cytokine receptor common beta chain as a potential activator of cytokine withdrawal-induced apoptosis.
Lee SF, Huang HM, Chao JR, Lin S, Yang-Yen HF, Yen JJ
Molecular and cellular biology 1999 Nov;19(11):7399-409
Molecular and cellular biology 1999 Nov;19(11):7399-409
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 97
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cell.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
