RQ5968

antibody from NSJ Bioreagents

Targeting: CSF2RB betaGMR, CD131, IL3RB, IL5RB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5968

Provider

NSJ Bioreagents

Product name

Il-3rb Antibody / Csf2rb / Cd131

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the Il-3rb antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse ANA-1 cells with Il-3rb antibody. Expected molecular weight: 99-130 kDa depending on glycosylation level.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with Il-3rb antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse ANA-1 cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat NRK cells with Il-3rb antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Il-3rb antibody.