BS3062

antibody from Bioworld Technology, Inc

Targeting: ACOT8 hACTE-III, hTE, NAP1, PTE-2, PTE1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS3062

Provider

Bioworld Technology, Inc

Product name

ACOT8 (P172) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 151-200 of Human ACOT8.

Description

ACOT8 (Acyl-CoA thioesterase 8) also designated, TEII p35 or hTE, is a novel human thioesterase that has been found to interact with the HIV protein Nef using yeast two-hybrid screening. Nef is an auxiliary gene of the human immunodeficiency virus (HIV) which facilitates virus replication and enhances infectivity. The roles of Nef in HIV-infected cells are likely to be mediated by specific interactions with cellular proteins. The interaction between Nef and ACOT8 is correlated with CD4 downregulation, suggesting that ACOT8 may be involved in Nef-mediated CD4 downregulation in HIV-infected cells. ACOT8 is 42% identical to thioesterase II from Escherichia coli, and it has no significant homology with the two types of animal thioesterases that have previously been cloned (type I and type II thioesterases).

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of ACOT8 (P172) pAb at 1:500 dilutionLane1:Hela cell lysateLane2:NIH-3T3 cell lysateLane3:sp2/0 cell lysateLane4:H9C2 cell lysate