MA5-15056

antibody from Invitrogen Antibodies

Targeting: FOSB AP-1, DKFZp686C0818, G0S3, GOS3, GOSB, MGC42291

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15056

Provider

Invitrogen Antibodies

Product name

FosB Monoclonal Antibody (H.234.10)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other Fos proteins, including c-fos, FRA1 and FRA2.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

H.234.10

Vial size

100 µL

Concentration

107 µg/mL

Storage

-20°C

References

Submitted references

Glutamate triggers the expression of functional ionotropic and metabotropic glutamate receptors in mast cells.

Alim MA, Grujic M, Ackerman PW, Kristiansson P, Eliasson P, Peterson M, Pejler G
Cellular & molecular immunology 2021 Oct;18(10):2383-2392

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear enriched cell extracts (30 µg lysate) of NIH/3T3 (Lane 1), NIH/3T3 Serum Starved (72 hours) (Lane 2), NIH/3T3 Serum Starved (72 hours) followed by Serum Release (10% serum for 4 hours) (Lane 3), HeLa (Lane 4), HeLa treated with EGF (25 ng/mL for 1 hour) (Lane 5) and Hep G2 (Lane 6). The blot was probed with Anti-FosB Monoclonal Antibody (H.234.10) (Product # MA5-15056, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 50 kDa band corresponding to FosB and a 40 kDa corresponding to FosB2 was observed across the cell lines tested and was enhanced upon treatment.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of FosB in HeLa cells using a FosB monoclonal antibody (Product # MA5-15056) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of FosB in paraffin-embedded human breast carcinoma using a FosB monoclonal antibody (Product # MA5-15056) in the presence of control peptide (left) or FosB blocking peptide (right).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Glutamate induces the expression of transcription factors and nuclear receptors. MCs (1 x 10 6 /ml) were exposed to glutamate (10, 100, or 500 uM) for 1 h, 4 h, or 24 h. a - e Relative mRNA levels (fold change) for Egr2, Egr3, Nr4a1, Nr4a3, and FosB were quantified by qPCR. The relative mRNA levels of targets were normalized to the housekeeping gene GAPDH. g Visualization of FosB protein in control- and glutamate-treated MCs using confocal microscopy. Cells were costained for FosB, tryptase (MC granule marker), and DAPI (nuclear marker). f Quantification of FosB staining in control and glutamate-treated MCs. Scale bars: 25 mum. Data represent mean values +- SEM ( n = 3). Significance was calculated with an unpaired t -test with Welch's correction. *** p

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 Colocalization of FosB, MC tryptase, and glutamate in an injured tendon. a Immunofluorescence image showing colocalization of FosB and MC tryptase (Mcpt6) in an injured Achilles tendon (AT). Note the abundance of cells double positive for tryptase (MCs) and FosB in the injured, but not in control, tissues. b , c Quantification of FosB and tryptase staining in the control and injured ATs. d Immunofluorescence images showing colocalization of FosB and glutamate in the control and injured ATs. Note the abundance of cells double positive for FosB and glutamate in the injured, but not in the control, ATs. e , f Quantification of FosB and glutamate staining in the control and injured tendons. * p