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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- M39-652R - Provider product page
- Provider
- SignalChem
- Product name
- Anti-phospho-p38 alpha (Thr180 Tyr182)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified by affinity chromatography
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian, Zebrafish
- Host
- Rabbit
- Epitope
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr180/Tyr182 conjugated to KLH
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39kDa p38 alpha protein phosphorylated at Thr180/Tyr182.
- Sample type
- Western Blot
Supportive validation
- Submitted by
- SignalChem (provider)
- Main image
- Experimental details
- Immunostaining of human breast cancer tissue showing p38 alpha protein when phosphorylated at Thr180/Tyr182 in red. Photo courtesy of Patsy Ruegg.
- Sample type
- Immunostaining
