- 36-8500 - Invitrogen Antibodies MAPK14 antibody

Submitted references SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats.

Muraleva NA, Stefanova NA, Kolosova NG
Antioxidants (Basel, Switzerland) 2020 Jul 28;9(8)

Methoxychlor and Vinclozolin Induce Rapid Changes in Intercellular and Intracellular Signaling in Liver Progenitor Cells.

Babica P, Zurabian R, Kumar ER, Chopra R, Mianecki MJ, Park JS, Jaša L, Trosko JE, Upham BL
Toxicological sciences : an official journal of the Society of Toxicology 2016 Sep;153(1):174-85

Opposing effects of membrane-anchored CX3CL1 on amyloid and tau pathologies via the p38 MAPK pathway.

Lee S, Xu G, Jay TR, Bhatta S, Kim KW, Jung S, Landreth GE, Ransohoff RM, Lamb BT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Sep 10;34(37):12538-46

Microglial derived tumor necrosis factor-α drives Alzheimer's disease-related neuronal cell cycle events.

Bhaskar K, Maphis N, Xu G, Varvel NH, Kokiko-Cochran ON, Weick JP, Staugaitis SM, Cardona A, Ransohoff RM, Herrup K, Lamb BT
Neurobiology of disease 2014 Feb;62:273-85

Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.

Jang IK, Cronshaw DG, Xie LK, Fang G, Zhang J, Oh H, Fu YX, Gu H, Zou Y
Proceedings of the National Academy of Sciences of the United States of America 2011 May 10;108(19):7926-31

T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.

Adachi K, Davis MM
Proceedings of the National Academy of Sciences of the United States of America 2011 Jan 25;108(4):1549-54

Cell death or survival promoted by alternative isoforms of ErbB4.

Sundvall M, Veikkolainen V, Kurppa K, Salah Z, Tvorogov D, van Zoelen EJ, Aqeilan R, Elenius K
Molecular biology of the cell 2010 Dec;21(23):4275-86

RhoA-GDP regulates RhoB protein stability. Potential involvement of RhoGDIalpha.

Ho TT, Merajver SD, Lapière CM, Nusgens BV, Deroanne CF
The Journal of biological chemistry 2008 Aug 1;283(31):21588-98

Selective activation of mitogen-activated protein (MAP) kinase kinase 3 and p38alpha MAP kinase is essential for cyclic AMP-dependent UCP1 expression in adipocytes.

Robidoux J, Cao W, Quan H, Daniel KW, Moukdar F, Bai X, Floering LM, Collins S
Molecular and cellular biology 2005 Jul;25(13):5466-79

p38 Mitogen-activated protein kinase plays a stimulatory role in hepatic gluconeogenesis.

Cao W, Collins QF, Becker TC, Robidoux J, Lupo EG Jr, Xiong Y, Daniel KW, Floering L, Collins S
The Journal of biological chemistry 2005 Dec 30;280(52):42731-7

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-p38 MAPK pThr180/pTyr182 in rat cell lysates using a Phospho-p38 MAPK pThr180/pTyr182 polyclonal antibody (Product # 36-8500).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1),HeLa treated with UV (40 min) (Lane 2), HeLa treated with TNF alpha (100 ng/mL for 10 min) (Lane 3), A549 (Lane 4) and A549 treated with UV (40 min) (Lane 5). The blot was probed with Anti-Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 38 kDa band corresponding to p38 MAPK alpha (Thr180, Tyr182) was observed across the cell lines tested and was enhanced upon treatment.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of p38 MAPK alpha in HeLa cell lysates that had been treated with UV (~254 nm) for 0 or 30 showing the specific immunolabeling of a ~39 kDa band corresponding to Phospho-p38 MAPK (Thr180, Tyr182) polyclonal antibody (Product # 36-8500).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed by loading 30 ug ME-180 Cas9 (Lane 1), ME-180 Cas9 treated with TNF-alpha (50 ng/ml for 20 minutes) (lane 2), ME-180 TRADD KO (Lane 3), ME-180 TRADD KO treated with TNF-alpha (50 ng/ml for 20 minutes) (Lane 4) whole cell extracts. The blots were probed with Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8) (Product # 36-8500, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Reduction in signal in CRISPR mediated knockout (KO) confirms that antibody is specific to Phospho-p38 MAPK alpha (Thr180, Tyr182) Monoclonal Antibody (F.52.8).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Phospho-p38 MAPK alpha (Thr180, Tyr182) was performed using 70% confluent log phase HeLa cells treated with UV for 40 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-p38 MAPK alpha (Thr180, Tyr182) Polyclonal Antibody (Product # 36-8500) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:100). Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of Alzheimer's disease brain tissue using p38 MAPK (pT180/pY182) Polyclonal Antibody, Rabbit

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 The protein content of p38 MAPK and p-p38 MAPK in the hippocampus of 20-day-old and 5- and 18-month-old Wistar and OXYS rats. ( a ) Representative western blots of total and phosphorylated p38 MAPK in the detergent-soluble and detergent-insoluble fractions from the hippocampus of Wistar and OXYS rats. Graphical presentation illustrates the relative protein content of p38 MAPK ( b ) and p-p38 MAPK ( c ) in Wistar and OXYS rats' hippocampi at different ages after normalization of the detergent-soluble fraction data to beta-actin and detergent-insoluble fraction data to GAPDH. Data are presented as mean +- SEM of five independent experiments. Immunostaining for p38 MAPK and p-p38 MAPK ( d ) in the hippocampus of 20-day-old and 5- and 18-month-old Wistar and OXYS rats. The nuclei were stained with DAPI (blue). Scale bars, 25 um. * Statistically significant differences between the strains of the same age; ^ significant differences from the previous age within a strain ( p < 0.05).

36-8500

Antibody data