Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324492 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal MAPK14 Antibody (Center T180/Y182)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal MAPK14 Antibody (Center T180/Y182)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MAPK14
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.38 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MAPK14 Antibody (Center T180/Y182) (Cat. #TA324492) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the MAPK14 antibody detected the MAPK14 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of A549 cell stained with MAPK14 Antibody (Center T180/Y182)(Cat#TA324492).A549 cells were incubated with MAPK14 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) .MAPK14 immunoreactivity is localized to Vesicles significantly and Cytoplasm weakly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MAPK14 Antibody (Center T180/Y182) (Cat. #TA324492)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAPK14 Antibody (Center T180/Y182) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
