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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- ARG55398 - Provider product page
- Provider
- Arigo
- Product name
- anti-p38 MAPK antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 295-324 (C-terminus) of Human p38 MAPK.
- Description
- Purification with Protein G.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: Jurkat cell lysate stained with ARG55398 anti-p38 MAPK antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 2 µg of 293 cell lysates either 1) nontransfected or 2) transiently transfected with the MAPK14 gene stained with ARG55398 anti-p38 MAPK antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cell stained with ARG55398 anti-p38 MAPK antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55398 anti-p38 MAPK antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
