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LearnF50496
antibody from NSJ Bioreagents
Targeting: MAPK14
CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F50496 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p38 Antibody (MAPK14)
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 295-324 from the human protein was used as the immunogen for this p38 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the p38 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- p38 antibody used in western blot to detect p38 in Jurkat cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of p38 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of p38 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 Phalloidin (red). DAPI was used as a nuclear counterstain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- p38 antibody flow cytometric analysis of HeLa cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-rabbit secondary Ab was used for the analysis.
