Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00136PU-N - Provider product page
- Provider
- OriGene
- Product name
- p38 (MAPK14) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 13D5, Purified
- Antibody type
- Monoclonal
- Description
- p38 (MAPK14) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 13D5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAPK14
- Isotype
- IgG
- Antibody clone number
- 13D5
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Detection of endogenous SAPK2alpha Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab SAPK2a-13D5 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2:SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF7; lane 6: MDA-MB231; lane 7: T47D
- Validation comment
- WB