MA5-35978

MAPK14 antibody from Invitrogen Antibodies

CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Immunohistochemistry

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Provider product page for MA5-35978

Antibody Data

Product number

MA5-35978

Provider

Invitrogen Antibodies

Product name

p38 MAPK alpha Monoclonal Antibody (07)

Provider product page

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This affinity purified antibody is directed against human EIF2A. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 86.7% homology with the immunizing sequence to mouse.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

07

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of p38 MAPK alpha using p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, Lane B: A431 whole cell lysate, Lane C: MCF7 whole cell lysate, Lane D: HepG2 whole cell lysate, (Lysates/proteins at 30 ?g per lane). The secondary antibody used was a Goat Anti-Mouse IgG (H+L)/HRP at 1:10000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa (We are unsure as to the identity of these extra bands).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of p38 MAPK alpha using p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, Lane B: A431 whole cell lysate, Lane C: MCF7 whole cell lysate, Lane D: HepG2 whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Mouse IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa (We are unsure as to the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of p38 MAPK alpha in Hela cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p38 MAPK alpha in formalin-fixed paraffin embedded sections human brain tissue sections using p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p38 MAPK alpha in formalin-fixed paraffin embedded sections human placenta tissue sections using p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of p38 MAPK alpha in formalin-fixed paraffin embedded sections human stomach tissue sections using p38 MAPK alpha Monoclonal Antibody (Product # MA5-35978) at a dilution of 1:100.