Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB8691 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Phospho-p38 alpha (T180/Y182) Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human p38 alpha when phosphorylated at T180 and Y182.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16539
- Isotype
- IgG
- Antibody clone number
- 696602
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Immunobiotics Beneficially Modulate TLR4 Signaling Triggered by Lipopolysaccharide and Reduce Hepatic Steatosis In Vitro.
The p38α Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1α.
Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model.
IL17A augments autophagy in Mycobacterium tuberculosis-infected monocytes from patients with active tuberculosis in association with the severity of the disease.
Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival.
Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts.
Kanmani P, Ansari A, Villena J, Kim H
Journal of immunology research 2019;2019:3876896
Journal of immunology research 2019;2019:3876896
The p38α Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1α.
Simões-Sousa S, Littler S, Thompson SL, Minshall P, Whalley H, Bakker B, Belkot K, Moralli D, Bronder D, Tighe A, Spierings DCJ, Bah N, Graham J, Nelson L, Green CM, Foijer F, Townsend PA, Taylor SS
Cell reports 2018 Oct 16;25(3):749-760.e6
Cell reports 2018 Oct 16;25(3):749-760.e6
Up-regulation of NHE8 by somatostatin ameliorates the diarrhea symptom in infectious colitis mice model.
Lei X, Cai L, Li X, Xu H, Geng C, Wang C
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2018 May;22(3):269-275
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2018 May;22(3):269-275
IL17A augments autophagy in Mycobacterium tuberculosis-infected monocytes from patients with active tuberculosis in association with the severity of the disease.
Tateosian NL, Pellegrini JM, Amiano NO, Rolandelli A, Casco N, Palmero DJ, Colombo MI, García VE
Autophagy 2017 Jul 3;13(7):1191-1204
Autophagy 2017 Jul 3;13(7):1191-1204
Regulatory module involving FGF13, miR-504, and p53 regulates ribosomal biogenesis and supports cancer cell survival.
Bublik DR, Bursać S, Sheffer M, Oršolić I, Shalit T, Tarcic O, Kotler E, Mouhadeb O, Hoffman Y, Fuchs G, Levin Y, Volarević S, Oren M
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 24;114(4):E496-E505
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 24;114(4):E496-E505
Photoprotective Effects of Cycloheterophyllin against UVA-Induced Damage and Oxidative Stress in Human Dermal Fibroblasts.
Huang CH, Li HJ, Wu NL, Hsiao CY, Lin CN, Chang HH, Hung CF
PloS one 2016;11(9):e0161767
PloS one 2016;11(9):e0161767
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Phospho-p38 alpha (T180/Y182) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 mJ/cm2 ultraviolet light (UV) followed by a 30 minute recovery. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-p38 alpha (T180/Y182) Monoclonal Antibody (Catalog # MAB8691) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-p38 alpha (T180/Y182) at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.